Isolation, Molecular Characterization and Growth Promoting Activity of Free Living Diazotrophs Screened from Soils of Lesotho

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dc.contributor.authorMonokoane, P. C.
dc.contributor.authorMekbib, Sissay Bekele
dc.contributor.authorHaiying, Li
dc.date.accessioned2016-11-30T16:24:56Z
dc.date.available2016-11-30T16:24:56Z
dc.date.issued2016-02
dc.description.abstractThirty six free living diazotrophic isolates represented from different soil pH (5 to 7.22) of Lesotho were characterized. A nitrogen free medium was used to screen nitrogen fixing diazotrophs. Strains were classified into three groups as Azospirillum, Azotobacter and Bacillus spp based on their biochemical and molecular characteristics. All the diazotrophic groups showed some degree of nitrogenase activity when assayed using acetylene (C2H2) reduction assay and production of Indole Acetic Acid (IAA) when screened for PGPR traits. Despite the strain variability, significantly higher nitrogenase activity (190.20 nmol/mL-1h-1) was exhibited by strain NUL3-2 (Azospirillum spp), which in contrary exhibited lower amount of IAA production (0.032 mg ml-1) compared to strain MAF7-1 (0.448 mg ml-1) from the group of Azotobacter spp. Further evaluation of strains efficacy for plant growth promotion activity is commendable under greenhouse experimentation for potential use of these strains as biofertilizer.en_ZA
dc.identifier.issn2315-7739
dc.identifier.uri10.15413/ajar.2015.0214
dc.identifier.urihttp://repository.tml.nul.ls/handle/20.500.14155/344
dc.language.isoenen_ZA
dc.publisherAcademia Publishingen_ZA
dc.subjectFree living N2 fixersen_ZA
dc.subjectAzosprillum spp.en_ZA
dc.subjectnitrogenase activityen_ZA
dc.subjectAzotobacter spp.en_ZA
dc.subjectBacillus spp.en_ZA
dc.titleIsolation, Molecular Characterization and Growth Promoting Activity of Free Living Diazotrophs Screened from Soils of Lesothoen_ZA
dc.typeArticleen_ZA
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